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rabbit  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit
    Rabbit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+uvrag/UVRAG+Antibody/pm41436430-132-72-91
    Average 93 stars, based on 38 article reviews
    rabbit - by Bioz Stars, 2026-09
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    other:

    Article Title: Bif-1c Attenuates Viral Proliferation by Regulating Autophagic Flux Blockade Induced by the Rabies Virus CVS-11 Strain in N2a Cells
    Article Snippet: The primary antibodies used were anti-ATG14 (no. 96752; Cell Signaling Technology), anti-UVRAG (no. 13115; Cell Signaling Technology), anti-BECN1 (no. 54101; Cell Signaling Technology), anti-p62 (P0067; Sigma-Aldrich), anti-LC3 (L8918; Sigma-Aldrich), anti-Bif1 (NBP2-24733; Novus), anti-GAPDH (RM2002; Beijing Ray Antibody Biotech), anti-RABV (5B12) (NB110-7542; Novus), and fluorescein isothiocyanate (FITC)-conjugated anti-RABV (800-092; FUJIREBIO).

    Article Title: TBC1D4 antagonizes RAB2A-mediated autophagic and endocytic pathways.
    Article Snippet: Macroautophagic/autophagic and endocytic pathways play essential roles in maintaining homeostasis at different levels.. It remains poorly understood how both pathways are coordinated and fine-tuned for proper lysosomal degradation of diverse cargoes.. We and others recently identified a Golgi-resident RAB GTPase, RAB2A, as a positive regulator that controls both autophagic and endocytic pathways.

    Article Title: Tumor-derived lactate promotes resistance to bevacizumab treatment by facilitating autophagy enhancer protein RUBCNL expression through histone H3 lysine 18 lactylation (H3K18la) in colorectal cancer.
    Article Snippet: Primary antibodies used in this study are used in this study are anti-Pan Kla (Jingjie, PTM-1401RM), anti-H3K18la (Jingjie, PTM-1406RM), anti-H3K9la (Jingjie, PTM-1419RM), anti-H4K8la (Jingjie, PTM-1415RM), antiH4K12la (Jingjie, PTM-1411RM), anti-HIF1A (Abcam, ab179483), anti-Histone H3 (Cell Signaling Technology, 4499), anti-LDHA (Proteintech, 19987–1-AP), anti-PARP (Cell Signaling Technology, 9532), anti-CASP3 (Cell Signaling Technology, 9662), anti-RUBCNL (Abcam, ab246954), anti-EP300 (Abcam, ab275378), anti-SQSTM1 (Cell Signaling Technology, 8025), anti-LC3 (Cell Signaling Technology, 3868), anti-FLAG tag (Proteintech, 60002–1-Ig), anti-HA tag (Proteintech, 51064–2-AP), anti-BECN1 (Proteintech, 11306–1-AP), anti-PIK3C3/VPS34 (Cell Signaling Technology, 4263), anti-UVRAG (Cell Signaling Technology, 13115) and anti-TUBB/β-tubulin (Proteintech, 10068–1-AP).

    Article Title: Bif-1c Attenuates Viral Proliferation by Regulating Autophagic Flux Blockade Induced by the Rabies Virus CVS-11 Strain in N2a Cells.
    Article Snippet: The primary antibodies used were anti-ATG14 (no. 96752; Cell Signaling Technology), anti-UVRAG (no. 13115; Cell Signaling Technology), anti-BECN1 (no. 54101; Cell Signaling Technology), anti-p62 (P0067; Sigma-Aldrich), anti-LC3 (L8918; Sigma-Aldrich), anti-Bif1 (NBP2-24733; Novus), anti-GAPDH (RM2002; Beijing Ray Antibody Biotech), anti-RABV (5B12) (NB110-7542; Novus), and fluorescein isothiocyanate (FITC)-conjugated anti-RABV (800-092; FUJIREBIO).

    Article Title: Tumor-derived lactate promotes resistance to bevacizumab treatment by facilitating autophagy enhancer protein RUBCNL expression through histone H3 lysine 18 lactylation (H3K18la) in colorectal cancer
    Article Snippet: Primary antibodies used in this study are used in this study are anti-Pan Kla (Jingjie, PTM-1401RM), anti-H3K18la (Jingjie, PTM-1406RM), anti-H3K9la (Jingjie, PTM-1419RM), anti-H4K8la (Jingjie, PTM-1415RM), anti-H4K12la (Jingjie, PTM-1411RM), anti-HIF1A (Abcam, ab179483), anti-Histone H3 (Cell Signaling Technology, 4499), anti-LDHA (Proteintech, 19987–1-AP), anti-PARP (Cell Signaling Technology, 9532), anti-CASP3 (Cell Signaling Technology, 9662), anti-RUBCNL (Abcam, ab246954), anti-EP300 (Abcam, ab275378), anti-SQSTM1 (Cell Signaling Technology, 8025), anti-LC3 (Cell Signaling Technology, 3868), anti-FLAG tag (Proteintech, 60002–1-Ig), anti-HA tag (Proteintech, 51064–2-AP), anti-BECN1 (Proteintech, 11306–1-AP), anti-PIK3C3/VPS34 (Cell Signaling Technology, 4263), anti-UVRAG (Cell Signaling Technology, 13115) and anti-TUBB/β-tubulin (Proteintech, 10068–1-AP).

    Article Title: DJ-1 binds to Rubicon to Impair LC-3 Associated Phagocytosis.
    Article Snippet: The following primary antibodies were used in this study: anti-DJ-1 (Cell Signalling Technology 5933, 1:1000); anti-β-actin (Sigma Aldrich A1978, 1:1000); anti-LC3-II (Abcam AB48394, 1:1000); anti-Beclin-1 (Cell Signalling Technology 3738, 1:1000); anti-UVRAG (Cell Signalling Technology 5320, 1:1000); anti-VPS34 (Cell Signalling Technology 4263, 1:1000); anti-Rubicon (Cell Signalling Technology 8465, 1:1000); anti-Atg5 (Cell Signalling Technology 12994, 1:1000); anti-Atg7 (Cell Signalling Technology 8558, 1:1000); antiGAPDH (Cell Signalling Technology 14C10, 1:1000); anti-p62 (Cell Signalling Technology 5114; 1:1000); anti-MBP (Santa Cruz SC13564, 1:1000); and anti-His (Santa Cruz SC-8036, 1:1000).

    Western Blot:

    Article Title: PDCD6 modulates LC3-associated phagocytosis-mediated antimicrobial defense by regulating lactate metabolism
    Article Snippet: TRIzol Reagent (R401-01) was from Vazyme. .. Antibodies for immunoblotting included anti-phospho-p40phox (Thr154) (4311), anti-phospho-AMPKα (Thr172) (2535S), anti-AMPKα (D63G4) (5832S), anti-phospho-S6 Ribosomal Protein (Ser235/236) (4856S), anti-S6 Ribosomal Protein (2217S), anti-phospho-p70 S6 Kinase (Thr389) (9234S), anti-p70 S6 Kinase (9202S), anti-Atg5 (12994), anti-Beclin-1 (3738), anti-Atg7 (8558), anti-Atg16L1 (8089), anti-LC3B (2775), anti-SQSTM1/p62 (5114), anti- PI3K (D9A5) (4263), anti-Rubicon (8465), anti-UVRAG (13115), anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (4370), anti-p44/42 MAPK (Erk1/2) (4695), antiphospho-JNK (Thr183/Tyr185) (4668), anti-JNK2 (9258), anti-phospho-p38 MAPK (Thr180/Tyr182) (9211), anti-p38 MAPK (8690), anti-phospho-IKKα/β (Ser176/180) (2697), anti-IKKβ (2678), anti-phosphoNF-κB p65 (Ser536) (3033), anti-NF-κB p65 (8242), anti-phospho-IκBα (Ser32) (2859), anti-IκBα (9242), anti-phospho-ULK1 (Ser757) (6888S) and anti-ULK1 (R600) (4773S) were from Cell Signaling Technology. .. Anti-Caspase-1 (p20) (AG-20B-0042) was from Adipogen.

    Immunofluorescence:

    Article Title: RAB5c controls the assembly of non-canonical autophagy machinery to promote phagosome maturation and microbicidal function of macrophages
    Article Snippet: .. Anti-RAB5c [Thermo Fisher Scientific, PA5-39408; IB, 1:2,000; Immunofluorescence (IF), 1:300], anti-GAPDH [Cell Signalling Technology (CST), Clone D16H11, 5174T, IB, 1:10,000], anti-β-actin (CST, Clone 8H10D10, 12262, IB, 1:5,000), anti-Microtubule-associated protein 1 light chain B (anti-MAPLC3B/LC3B; CST, 27759, IB, 1:1,000), anti-LC3 (LC3A/LC3B/LC3C; MBL Int., PM036, IF: 1:250), anti-RAB5a (CST, 2143, IB, 1:1,000), anti-RAB5b (Santa Cruz, Clone F-9, sc373725, IB, 1:1,000), anti-EEA1 (CST, Clone C45B10, 3288, IB, 1:1,000), anti-UNC93B (Abcam, 69497, IB, 1:3,000), anti-GM130 (MBL, Clone 5G8, M1793, IB, 1:500), anti-UVRAG (CST, Clone D2Q1Z, 13115, IB, 1:1,000), anti-Phosphatidylinositol 3-kinase catalytic subunit type 3 (anti-PIK3C3/VPS34; Clone D9A5, CST 4263, IB, 1:1,000), anti-Beclin1 (CST, 3738, IB, 1:1,000), anti-Rubicon (Clone D9F7, CST, 8465, IB, 1:1,000), anti-p-p47 phox (S328) (Abcam, 111855, IF, 1:50), anti-p47 phox (Abcam, Clone erp27205, 308256, IB, 1:1,000; IF, 1:250), anti-p-p40 phox (Thr514) (CST, 4311, IB, 1:1,000), anti-p22 phox (Abcam, 75941 IB, 1:1,000), anti-gp91 phox (BD Bioscience, Clone53, 611414, IB, 1:2,000), anti-ERK1/2 (CST, Clone 1375F, 4695, IF, 1:500), anti-p-ERK1/2(Thr202/204) (CST, Clone 197G2, 9106, IF, 1:100), anti-ATP6V0d1 (Abcam, Clone erp18320-38, IB, 1:1,000), anti-ATP6V1A (Abcam, Clone erp19270, 199326, IB, 1:4,000; IF, 1:250), anti-ATG16L1 (MBL, Clone 1F12, M150-3, IB, 1:1,000), anti-ATG5/12 (CST, Clone D5F5U, 12994, IB, 1:1,000), anti-LAMP1 (CST, Clone E5N9Z, #99437, IF, 1:150), Alexa Fluor 647 polyclonal goat anti-rabbit IgG (Thermo Fisher Scientific, A21244, IF: 1:750), Alexa Fluor 488 polyclonal donkey anti-mouse IgG (Thermo Fisher Scientific, A21202, IF: 1:750), Alexa Fluor 647 polyclonal donkey anti-mouse IgG (Thermo Fisher Scientific, A31571, IF: 1:750), HRP-conjugated anti-rabbit IgG (CST, 7074, IB: 1:5,000), and HRP-conjugated anti-mouse IgG (CST, 7076, IB: 1:5,000) were used as indicated in manufactures′ datasheets. ..



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    93
    Proteintech anti uvrag
    (A) The expression levels <t>of</t> <t>LC3B</t> and P62 in T24 cells transfected with vector, Flag-Rubicon-L, or Flag-Rubicon-S were detected by western blotting. (B) T24 cells were transfected with vector, Flag-Rubicon-L or Flag-Rubicon-S plasmids, and those co-transfected with mCherry-EGFP-LC3B. The autophagosomes with yellow puncta and autolysosomes with red puncta. Bar: 10 μm. (C) T24 cells were transfected with the vector, Flag-Rubicon-L, and Flag-Rubicon-S plasmids, and then co-cultured with CD8 + T cells, and cell viability was measured by CCK-8. (D) Western blotting with the indicated antibodies in T24 cells transfected with scramble, sh-RUBCN-L or sh-RUBCN-S. The efficiency of RUBCN-L knockdown and RUBCN-S knockdown in T24 cells was detected by agarose gel electrophoresis (left) and qRT-PCR (right). (E) Co-IP assay using antibody specific for Rubicon showed that Rubicon interacted with <t>UVRAG</t> and Beclin1 in T24 cells. The precipitate was subjected to western blotting with the antibodies against Rubicon, UVRAG, and Beclin1. (F) Co-IP assay using antibody specific for Flag showed that Flag-Rubicon-S interacted with UVRAG and Beclin1(Right), while Flag-Rubicon-L could not bind to UVRAG and Beclin1 in T24 cells (Left). The precipitate was subjected to western blotting with the antibodies against Flag, UVRAG, and Beclin1. (G) The expression levels of LC3B and P62 in CUL5-KO T24 cells were detected by western blotting. (H) CUL5-WT, CUL5-KO#1, and CUL5-KO#2 T24 cells were transfected with the mCherry-EGFP-LC3B. The autophagosomes with yellow puncta and autolysosomes with red puncta. Bar: 10 μm. (I) Western blotting with the indicated antibodies in CUL5-WT and CUL5-KO T24 cells transfected with scramble or sh-PTBP1#1. (J) Western blotting with the indicated antibodies in CUL5-WT and CUL5-KO T24 cells transfected with scramble or sh-RUBCN-L, and agarose gel electrophoresis for analysis of RUBCN isoforms. (K) CUL5-WT, CUL5-KO#1, and CUL5-KO#2 T24 cells were transfected with the scramble or sh-RUBCN-L, and then co-cultured with CD8 + T cells, and cell viability was measured by CCK-8. (L) Western blotting with the indicated antibodies in CUL5-WT and CUL5-KO T24 cells transfected with scramble or sh-RUBCN-S, and agarose gel electrophoresis for analysis of RUBCN isoforms. (M) CUL5-WT, CUL5-KO#1, and CUL5-KO#2 T24 cells were transfected with the scramble or sh-RUBCN-S, and then co-cultured with CD8 + T cells, and cell viability was measured by CCK-8. (N) The expression levels of MHC-I (HLA-A, -B, -C) in CUL5-KO T24 cells co-cultured with CD8 + T cells were detected by western blotting. Data are presented as the means ± SD from three independent experiments. Student t test was applied to analyze and compare the data in C, D, K, and M. ns, nonsignificant; ** P < 0.01; *** P < 0.001. The raw data underlying all figures can be found in . Original blots and gels can be found in .
    Anti Uvrag, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti uvrag  (Cell Signaling Technology Inc)
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    (A) The expression levels <t>of</t> <t>LC3B</t> and P62 in T24 cells transfected with vector, Flag-Rubicon-L, or Flag-Rubicon-S were detected by western blotting. (B) T24 cells were transfected with vector, Flag-Rubicon-L or Flag-Rubicon-S plasmids, and those co-transfected with mCherry-EGFP-LC3B. The autophagosomes with yellow puncta and autolysosomes with red puncta. Bar: 10 μm. (C) T24 cells were transfected with the vector, Flag-Rubicon-L, and Flag-Rubicon-S plasmids, and then co-cultured with CD8 + T cells, and cell viability was measured by CCK-8. (D) Western blotting with the indicated antibodies in T24 cells transfected with scramble, sh-RUBCN-L or sh-RUBCN-S. The efficiency of RUBCN-L knockdown and RUBCN-S knockdown in T24 cells was detected by agarose gel electrophoresis (left) and qRT-PCR (right). (E) Co-IP assay using antibody specific for Rubicon showed that Rubicon interacted with <t>UVRAG</t> and Beclin1 in T24 cells. The precipitate was subjected to western blotting with the antibodies against Rubicon, UVRAG, and Beclin1. (F) Co-IP assay using antibody specific for Flag showed that Flag-Rubicon-S interacted with UVRAG and Beclin1(Right), while Flag-Rubicon-L could not bind to UVRAG and Beclin1 in T24 cells (Left). The precipitate was subjected to western blotting with the antibodies against Flag, UVRAG, and Beclin1. (G) The expression levels of LC3B and P62 in CUL5-KO T24 cells were detected by western blotting. (H) CUL5-WT, CUL5-KO#1, and CUL5-KO#2 T24 cells were transfected with the mCherry-EGFP-LC3B. The autophagosomes with yellow puncta and autolysosomes with red puncta. Bar: 10 μm. (I) Western blotting with the indicated antibodies in CUL5-WT and CUL5-KO T24 cells transfected with scramble or sh-PTBP1#1. (J) Western blotting with the indicated antibodies in CUL5-WT and CUL5-KO T24 cells transfected with scramble or sh-RUBCN-L, and agarose gel electrophoresis for analysis of RUBCN isoforms. (K) CUL5-WT, CUL5-KO#1, and CUL5-KO#2 T24 cells were transfected with the scramble or sh-RUBCN-L, and then co-cultured with CD8 + T cells, and cell viability was measured by CCK-8. (L) Western blotting with the indicated antibodies in CUL5-WT and CUL5-KO T24 cells transfected with scramble or sh-RUBCN-S, and agarose gel electrophoresis for analysis of RUBCN isoforms. (M) CUL5-WT, CUL5-KO#1, and CUL5-KO#2 T24 cells were transfected with the scramble or sh-RUBCN-S, and then co-cultured with CD8 + T cells, and cell viability was measured by CCK-8. (N) The expression levels of MHC-I (HLA-A, -B, -C) in CUL5-KO T24 cells co-cultured with CD8 + T cells were detected by western blotting. Data are presented as the means ± SD from three independent experiments. Student t test was applied to analyze and compare the data in C, D, K, and M. ns, nonsignificant; ** P < 0.01; *** P < 0.001. The raw data underlying all figures can be found in . Original blots and gels can be found in .
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    (A) The expression levels of LC3B and P62 in T24 cells transfected with vector, Flag-Rubicon-L, or Flag-Rubicon-S were detected by western blotting. (B) T24 cells were transfected with vector, Flag-Rubicon-L or Flag-Rubicon-S plasmids, and those co-transfected with mCherry-EGFP-LC3B. The autophagosomes with yellow puncta and autolysosomes with red puncta. Bar: 10 μm. (C) T24 cells were transfected with the vector, Flag-Rubicon-L, and Flag-Rubicon-S plasmids, and then co-cultured with CD8 + T cells, and cell viability was measured by CCK-8. (D) Western blotting with the indicated antibodies in T24 cells transfected with scramble, sh-RUBCN-L or sh-RUBCN-S. The efficiency of RUBCN-L knockdown and RUBCN-S knockdown in T24 cells was detected by agarose gel electrophoresis (left) and qRT-PCR (right). (E) Co-IP assay using antibody specific for Rubicon showed that Rubicon interacted with UVRAG and Beclin1 in T24 cells. The precipitate was subjected to western blotting with the antibodies against Rubicon, UVRAG, and Beclin1. (F) Co-IP assay using antibody specific for Flag showed that Flag-Rubicon-S interacted with UVRAG and Beclin1(Right), while Flag-Rubicon-L could not bind to UVRAG and Beclin1 in T24 cells (Left). The precipitate was subjected to western blotting with the antibodies against Flag, UVRAG, and Beclin1. (G) The expression levels of LC3B and P62 in CUL5-KO T24 cells were detected by western blotting. (H) CUL5-WT, CUL5-KO#1, and CUL5-KO#2 T24 cells were transfected with the mCherry-EGFP-LC3B. The autophagosomes with yellow puncta and autolysosomes with red puncta. Bar: 10 μm. (I) Western blotting with the indicated antibodies in CUL5-WT and CUL5-KO T24 cells transfected with scramble or sh-PTBP1#1. (J) Western blotting with the indicated antibodies in CUL5-WT and CUL5-KO T24 cells transfected with scramble or sh-RUBCN-L, and agarose gel electrophoresis for analysis of RUBCN isoforms. (K) CUL5-WT, CUL5-KO#1, and CUL5-KO#2 T24 cells were transfected with the scramble or sh-RUBCN-L, and then co-cultured with CD8 + T cells, and cell viability was measured by CCK-8. (L) Western blotting with the indicated antibodies in CUL5-WT and CUL5-KO T24 cells transfected with scramble or sh-RUBCN-S, and agarose gel electrophoresis for analysis of RUBCN isoforms. (M) CUL5-WT, CUL5-KO#1, and CUL5-KO#2 T24 cells were transfected with the scramble or sh-RUBCN-S, and then co-cultured with CD8 + T cells, and cell viability was measured by CCK-8. (N) The expression levels of MHC-I (HLA-A, -B, -C) in CUL5-KO T24 cells co-cultured with CD8 + T cells were detected by western blotting. Data are presented as the means ± SD from three independent experiments. Student t test was applied to analyze and compare the data in C, D, K, and M. ns, nonsignificant; ** P < 0.01; *** P < 0.001. The raw data underlying all figures can be found in . Original blots and gels can be found in .

    Journal: PLOS Biology

    Article Title: CUL5 E3 ubiquitin ligase regulates the evasion of bladder cancer cells to CD8 + T cell-mediated killing by inhibiting autophagy

    doi: 10.1371/journal.pbio.3003647

    Figure Lengend Snippet: (A) The expression levels of LC3B and P62 in T24 cells transfected with vector, Flag-Rubicon-L, or Flag-Rubicon-S were detected by western blotting. (B) T24 cells were transfected with vector, Flag-Rubicon-L or Flag-Rubicon-S plasmids, and those co-transfected with mCherry-EGFP-LC3B. The autophagosomes with yellow puncta and autolysosomes with red puncta. Bar: 10 μm. (C) T24 cells were transfected with the vector, Flag-Rubicon-L, and Flag-Rubicon-S plasmids, and then co-cultured with CD8 + T cells, and cell viability was measured by CCK-8. (D) Western blotting with the indicated antibodies in T24 cells transfected with scramble, sh-RUBCN-L or sh-RUBCN-S. The efficiency of RUBCN-L knockdown and RUBCN-S knockdown in T24 cells was detected by agarose gel electrophoresis (left) and qRT-PCR (right). (E) Co-IP assay using antibody specific for Rubicon showed that Rubicon interacted with UVRAG and Beclin1 in T24 cells. The precipitate was subjected to western blotting with the antibodies against Rubicon, UVRAG, and Beclin1. (F) Co-IP assay using antibody specific for Flag showed that Flag-Rubicon-S interacted with UVRAG and Beclin1(Right), while Flag-Rubicon-L could not bind to UVRAG and Beclin1 in T24 cells (Left). The precipitate was subjected to western blotting with the antibodies against Flag, UVRAG, and Beclin1. (G) The expression levels of LC3B and P62 in CUL5-KO T24 cells were detected by western blotting. (H) CUL5-WT, CUL5-KO#1, and CUL5-KO#2 T24 cells were transfected with the mCherry-EGFP-LC3B. The autophagosomes with yellow puncta and autolysosomes with red puncta. Bar: 10 μm. (I) Western blotting with the indicated antibodies in CUL5-WT and CUL5-KO T24 cells transfected with scramble or sh-PTBP1#1. (J) Western blotting with the indicated antibodies in CUL5-WT and CUL5-KO T24 cells transfected with scramble or sh-RUBCN-L, and agarose gel electrophoresis for analysis of RUBCN isoforms. (K) CUL5-WT, CUL5-KO#1, and CUL5-KO#2 T24 cells were transfected with the scramble or sh-RUBCN-L, and then co-cultured with CD8 + T cells, and cell viability was measured by CCK-8. (L) Western blotting with the indicated antibodies in CUL5-WT and CUL5-KO T24 cells transfected with scramble or sh-RUBCN-S, and agarose gel electrophoresis for analysis of RUBCN isoforms. (M) CUL5-WT, CUL5-KO#1, and CUL5-KO#2 T24 cells were transfected with the scramble or sh-RUBCN-S, and then co-cultured with CD8 + T cells, and cell viability was measured by CCK-8. (N) The expression levels of MHC-I (HLA-A, -B, -C) in CUL5-KO T24 cells co-cultured with CD8 + T cells were detected by western blotting. Data are presented as the means ± SD from three independent experiments. Student t test was applied to analyze and compare the data in C, D, K, and M. ns, nonsignificant; ** P < 0.01; *** P < 0.001. The raw data underlying all figures can be found in . Original blots and gels can be found in .

    Article Snippet: Antibodies used included primary antibodies against CUL5 (Abclonal, A5369), β-Actin (Proteintech, 66009-1-Ig), PRMT5 (Proteintech, 18436-1-AP), THOC2 (Proteintech, 55178-1-AP), THRAP3 (Proteintech, 19744-1-AP), SNRNP200 (Proteintech, 23875-1-AP), PTBP1 (Proteintech, 12582-1-AP), SF3B1(Proteintech, 27684-1-AP), PRPF8(Proteintech, 11171-1-AP), SF3B2 (Proteintech, 10919-1-AP), HNRNPC (Proteintech, 11760-1-AP), LC3B (Abclonal, A5618), P62 (Proteintech, 18420-1-AP), Rubicon (Proteintech, 21444-1-AP), UVRAG (Proteintech, 29190-1-AP), Beclin1 (Proteintech, 11306-1-AP), HLA-class I (Proteintech, 15240-1-AP), Rabbit control IgG (Abclonal, AC005), Mouse control IgG (Abclonal, AC011), Mouse anti-HA tag (Abclonal, AE008), Rabbit anti-HA tag (Abclonal, AE036), Mouse anti-Flag tag (Abclonal, AE005), and Rabbit anti-Flag tag (Abclonal, AE004); HRP-conjugated secondary goat anti-mouse (Proteintech, SA00001-1), or goat anti-rabbit (Proteintech, SA00001-2) antibodies.

    Techniques: Expressing, Transfection, Plasmid Preparation, Western Blot, Cell Culture, CCK-8 Assay, Knockdown, Agarose Gel Electrophoresis, Quantitative RT-PCR, Co-Immunoprecipitation Assay